GSJ: Received Apr. 16, 2007: http://wbabin.net/saba/saba72.htm

A new an apparently significant advance in DNA sequencing is pyrosequencing using microspheres and a fiber-optic picotiter plate (1,2,3).

It has been recognized, and claimed herein, that the this technology is perfectly suited to identify ligands which alter the functioning of polymerases (such as HIV reverse transcriptase) and other proteins involved in nucleic acid polymerization.

Figure 1 delineates the process, identifying inhibitors.

Various means of encoding beads (including microspheres) are well known, as are methods of synthesizing libraries of compounds on such beads. Alternatively to encoding the bead, a library member could be directly identified such as by mass spectrometry.

The molecular library attached to such beads could be of any class, such as small organics, peptides, or oligonucleotides. Library may be optionally dissociated from the beads by incorporation of an appropriate linker.

Notice that activators as well as inhibitors could be identified, and that allosteric modulators of known activators or inhibitors could be identified.

If it should be that the above invention is indeed novel any patentable rights I may have, I freely give away.

It is hoped that others will honor the invention as delineated above and by the following claims.

Claims